Unraveling novel crosstalk between distinct neutrophil subsets and soluble plasma factors in sepsis and ARDS

Abstract Sepsis is a life-threatening condition caused by an aberrant immune response to infection and subsequent organ dysfunction. Further, due shared mechanisms such as severe inflammation endothelial dysfunction, sepsis remains the most common etiology in acute respiratory distress syndrome (ARDS) patients. Despite multiple efforts identify distinct signature support development of new therapeutics, significant unmet clinical need. While singular components dysregulated have been explored, studies linking different data layers are lacking. Using integrated systems immunology approach, we evaluated neutrophil phenotypes related changes soluble plasma factors versus healthy donors. Results from our differentially expressed mature immature subsets show that expression CD10 associated with pentraxin-3, angiopoietin-2, lysophosphatidylcholines, cohort. These findings detail novel correlation between increased presence neutrophils dysfunction/metabolic alterations Additionally, profiled landscape levels factors, subset ARDS patients Here, present reveal crosstalk parameters indicative Overall, study reinforces importance integration provides insights could help inform future mechanistic studies, early patient stratification treatment options.